Reagents
IRSF encourages investigators to share reagents. If you have an animal model, expression plasmid, antibodies, permanent cell lines, assay or other reagent you would like to make available to the Rett scientific community please contact This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

Antibodies


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Millipore is a Life Science leader providing cutting-edge technologies, tools and services for bioscience research and biopharmaceutical manufacturing. They offer an extensive portfolio of products and services in: Life Sciences, Drug Discovery and Development, Lab Filtration, Lab Water, Upstream Bioprocessing, Downstream Bioprocessing and Process Monitoring. Click here to search their wide selection of available antibodies or to browse antibodies by category. Millipore’s corporate headquarters are located in Billerica, MA.

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Santa Cruz Biotechnology, Inc. is focused on the ongoing development of research antibodies. They seek to continue to offer the broadest range of research reagents in the field by providing superior, innovative primary antibodies and support products. Its international headquarters are located in Santa Cruz, CA.


National Institute on Aging, NIH
Weidong Wang has developed a polyclonal MeCP2 antibody generated in rabbits. The antibody is suitable for immunoprecipitation and immunoblotting of MeCP2 from human, mouse and rat. For more information please contact This e-mail address is being protected from spam bots, you need JavaScript enabled to view it .

University of California, Davis – La Salle Lab
Janine LaSalle has developed a C-terminal chicken anti-MeCP2 antibody. For more information please contact Dr. LaSalle: This e-mail address is being protected from spam bots, you need JavaScript enabled to view it


Expression Vectors


University of Massachusetts, Amherst
Christopher Woodcock has made mutant human MeCP2 plasmids available. All have been sequenced and produce good yields of protein. Please contact This e-mail address is being protected from spam bots, you need JavaScript enabled to view it for the protocols. The following plasmids are available:

Wildtype
E397K
E397K
F155S
R106W
R294X
H370X
R453X
R106W + R294X
R106W + H370X
T158M
R133C
F155S


Universita dell'Insubria, Italy

The laboratory of Charlotte Kilstrup-Nielsen and Nicoletta Landsberger has different expression vectors for prokaryotic and mammalian cells of wild type and mutated MeCP2 from human, mouse and Xenopus. A more detailed description will follow. In the interim, for more information please contact This e-mail address is being protected from spam bots, you need JavaScript enabled to view it .
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